The coat protein gene of isolates of citrus tristeza virus (CTV) from 20 citrus-producing
regions around the world was amplified by RT-PCR, TA cloned, and characterized by SSCP.
Haplotypes that produced different patterns within each geographic region were sequenced
and a database of 153 accessions of CTV was assembled. Phylogenetic analysis revealed
the existence of seven well-defined clusters (Coefficient of differentiation 0.78). An
asymmetric PCR-ELISA typing (APET) assay was developed in the frame of this clustering …